Article
Comparison of CRISPR and Marker-Based Methods for the Engineering of Phage T7.
Viruses - 10 Feb 2020
Grigonyte Aurelija M, Harrison Christian, MacDonald Paul R, Montero-Blay Ariadna, Tridgett Matthew, Duncan John, Sagona Antonia P, Constantinidou Chrystala, Jaramillo Alfonso, Millard Andrew
Abstract excerpt
With the recent rise in interest in using lytic bacteriophages as therapeutic agents, there is an urgent requirement to understand their fundamental biology to enable the engineering of their genomes. Current methods of phage engineering rely on homologous recombination, followed by a system of selection to identify recombinant phages. For bacteriophage T7, the host genes cmk or trxA have been used as a selection...
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