Targeted Transition Mutations in Phage-Assisted Evolution.

Abstract excerpt

Phage-assisted evolution is a powerful strategy for directed evolution in which the protein undergoes continuous mutagenesis under selective pressure to rapidly evolve protein variants. However, conventional approaches often introduce mutations across the entire target plasmid, not just the gene-of-interest (GOI), leading to unwanted genomic changes that enable the evasion of selective pressure. To overcome this...

Topics

• Directed Molecular Evolution
• Mutation
• Mutagenesis
• Bacteriophages
• Bacteriophage M13
• Promoter Regions, Genetic
• Plasmids