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Article

Repurposing an Endogenous CRISPR-Cas System to Generate and Study Subtle Mutations in Bacteriophages.

The CRISPR journal - 1 Dec 2024

Kamata Kotaro, Birkholz Nils, Ceelen Marijn, Fagerlund Robert D, Jackson Simon A, Fineran Peter C

Abstract excerpt

While bacteriophage applications benefit from effective phage engineering, selecting the desired genotype after subtle modifications remains challenging. Here, we describe a two-phase endogenous CRISPR-Cas-based phage engineering approach that enables selection of small defined edits in Pectobacterium carotovorum phage ZF40. We designed plasmids containing sequences homologous to ZF40 and a mini-CRISPR array. The...

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Identifiers

PMID: 39347602
DOI: 10.1089/crispr.2024.0047

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