Article
Analyses of spontaneous mutations of cloned gene 49 of phage T4.
Mutation research - 20 Feb 2001
Hartung M, Slack M, Kemper B
Abstract excerpt
Holliday structure resolving enzyme endonuclease VII (endo VII) of phage T4 is highly toxic for E. coli when expressed outside of the phage infection environment. As a consequence, plasmids with a mutated gene 49, the gene which encodes for endo VII, can be easily isolated and characterised. We have isolated and characterised 400 survivors from independent transformations with a plasmid carrying gene 49 under the...
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