Article
Precise genome engineering in Pseudomonas using phage-encoded homologous recombination and the Cascade-Cas3 system.
Nature protocols - 1 Sept 2023
Zheng Wentao, Xia Yandong, Wang Xue, Gao Shiqing, Zhou Diao, Ravichandran Vinothkannan, Jiang Chanjuan, Tu Qiang, Yin Yulong, Zhang Youming, Fu Jun, Li Ruijuan, Yin Jia
Abstract excerpt
A lack of generic and effective genetic manipulation methods for Pseudomonas has restricted fundamental research and utilization of this genus for biotechnology applications. Phage-encoded homologous recombination (PEHR) is an efficient tool for bacterial genome engineering. This PEHR system is based on a lambda Red-like operon (BAS) from Pseudomonas aeruginosa phage Ab31 and a Rac bacteriophage RecET-like operon...
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