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Article

An efficient, scarless, selection-free technology for phage engineering.

RNA biology - 1 Jan 2023

Goren Moran G, Mahata Tridib, Qimron Udi

Abstract excerpt

Most recently developed phage engineering technologies are based on the CRISPR-Cas system. Here, we present a non-CRISPR-based method for genetically engineering the Escherichia coli phages T5, T7, P1, and λ by adapting the pORTMAGE technology, which was developed for engineering bacterial genomes. The technology comprises E. coli harbouring a plasmid encoding a potent recombinase and a gene transiently silencing...

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Identifiers

PMID: 37846029
DOI: 10.1080/15476286.2023.2270344
PMCID: PMC10583621

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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