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Article

A TALEN-based strategy for efficient bi-allelic miRNA ablation in human cells.

RNA (New York, N.Y.) - 1 Jun 2014

Uhde-Stone Claudia, Sarkar Nandita, Antes Travis, Otoc Nicole, Kim Young, Jiang Yan J, Lu Biao

Abstract excerpt

Significant progress in the functional understanding of microRNAs (miRNAs) has been made in mice, but a need remains to develop efficient tools for bi-allelic knockouts of miRNA in the human genome. Transcription activator-like effector nucleases (TALENs) provide an exciting platform for targeted gene ablation in cultured human cells, but bi-allelic modifications induced by TALENs alone occur at low frequency,...

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Identifiers

PMID: 24717974
DOI: 10.1261/rna.042010.113
PMCID: PMC4024647

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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