Article
In vivo CRISPR screening for phenotypic targets of the mir-35-42 family in C. elegans.
Genes & development - 1 Sept 2020
Yang Bing, Schwartz Matthew, McJunkin Katherine
Abstract excerpt
Identifying miRNA target genes is difficult, and delineating which targets are the most biologically important is even more difficult. We devised a novel strategy to test the phenotypic impact of individual microRNA-target interactions by disrupting each predicted miRNA-binding site by CRISPR-Cas9 genome editing in C. elegans We developed a multiplexed negative selection screening approach in which edited loci...
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