Article
Somatic mosaicism and allele complexity induced by CRISPR/Cas9 RNA injections in mouse zygotes.
Developmental biology - 1 Sept 2014
Yen Shuo-Ting, Zhang Min, Deng Jian Min, Usman Shireen J, Smith Chad N, Parker-Thornburg Jan, Swinton Paul G, Martin James F, Behringer Richard R
Abstract excerpt
Tyrosinase is the rate-limiting enzyme for the production of melanin pigmentation. In the mouse and other animals, homozygous null mutations in the Tyrosinase gene (Tyr) result in the absence of pigmentation, i.e. albinism. Here we used the CRISPR/Cas9 system to generate mono- and bi-allelic null mutations in the Tyr locus by zygote injection of two single-guide and Cas9 RNAs. Injection into C57BL/6N wild-type...
Topics
- Albinism
- Alleles
- Animals
- Bacterial Proteins
- Base Sequence
- CRISPR-Associated Protein 9
- CRISPR-Associated Proteins
- CRISPR-Cas Systems
- Endonucleases
- Female
- Gene Editing
- High-Throughput Nucleotide Sequencing
- Male