Article
Highly efficient base editing with expanded targeting scope using SpCas9-NG in rabbits.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology - 1 Jan 2020
Liu Zhiquan, Shan Huanhuan, Chen Siyu, Chen Mao, Song Yuning, Lai Liangxue, Li Zhanjun
Abstract excerpt
Base editors, composed of a cytidine deaminase or an evolved adenine deaminase fused to Cas9 nickase, enable efficient C-to-T or A-to-G conversion in various organisms. However, the NGG protospacer adjacent motif (PAM) requirement of Streptococcus pyogenes Cas9 (SpCas9) substantially limits the target sites suitable for base editing. Quite recently, a new engineered SpCas9-NG variant, which can recognize minimal...
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