Article
Disease modeling by efficient genome editing using a near PAM-less base editor <i>in vivo</i>
2021-06-29
Abstract excerpt
Base Editors are emerging as an innovative technology to introduce point mutations in complex genomes. So far, the requirement of an NGG Protospacer Adjacent Motif (PAM) at a suitable position often limits the editing possibility to model human pathological mutations in animals. Here we show that, using the CBE4max-SpRY variant recognizing the NRN PAM sequence, we could introduce point mutations for the first time...
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Identifiers and source
- Literature Corpus work
- 87fa6b2f-211e-59ad-a366-8fffc4e28ade
- DOI
- 10.1101/2021.06.28.450169