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Article

Ligand-activated BRET9 imaging for measuring protein-protein interactions in living mice.

Chemical communications (Cambridge, England) - 19 Dec 2019

Bae Kim Sung, Fujii Rika, Natarajan Arutselvan, Massoud Tarik F, Paulmurugan Ramasamy

Abstract excerpt

Bioluminescence resonance energy transfer (BRET) is a commonly used assay system for studying protein-protein interactions and protein folding in vivo. Conventional BRET systems have solely depended on an overlap of the energy donor and acceptor spectra. In this study, we engineered a conceptually unique ligand-activatable BRET system (termed BRET9), where a full-length Artificial Luciferase variant 23 (ALuc23),...

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PMID: 31807738
DOI: 10.1039/c9cc07634d

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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