Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A new protein-protein interaction sensor based on tripartite split-GFP association.

Scientific reports - 4 Oct 2013

Cabantous Stéphanie, Nguyen Hau B, Pedelacq Jean-Denis, Koraïchi Faten, Chaudhary Anu, Ganguly Kumkum, Lockard Meghan A, Favre Gilles, Terwilliger Thomas C, Waldo Geoffrey S

Abstract excerpt

Monitoring protein-protein interactions in living cells is key to unraveling their roles in numerous cellular processes and various diseases. Previously described split-GFP based sensors suffer from poor folding and/or self-assembly background fluorescence. Here, we have engineered a micro-tagging system to monitor protein-protein interactions in vivo and in vitro. The assay is based on tripartite association...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Gene Order
Share to Genetic Vectors

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Molecular Imaging

Share to Protein Engineering

Share to Protein Interaction Mapping

Share to Protein Multimerization

Share to Solubility

Share to Tacrolimus Binding Proteins

Source

Open in PubMed Open full text

Identifiers

PMID: 24092409
DOI: 10.1038/srep02854
PMCID: PMC3790201

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A new protein-protein interaction sensor based on tripartite split-GFP association.PMID 24092409Read ↗

Efficiently folding and circularly permuted variants of the Sapphire mutant of GFP.Semantic proximity Read ↗

Rational design and implementation of a chemically inducible heterotrimerization system.Semantic proximity Read ↗

Split T7 RNA polymerase biosensors to study multiprotein interaction dynamics.Semantic proximity Read ↗

Adaptable, Turn-On Monobody (ATOM) Fluorescent Biosensors for Multiplexed Detection in CellsSemantic proximity Read ↗

Protein stabilization with retained function of monellin using a split GFP system.Semantic proximity Read ↗

A precise and general FRET-based method for monitoring structural transitions in protein self-organizationSemantic proximity Read ↗

Principles and applications of green fluorescent protein-based biosensors: a mini-review.Semantic proximity Read ↗

Ligand-activated BRET9 imaging for measuring protein-protein interactions in living mice.Semantic proximity Read ↗

In vivo and in vitro protein solubility assays using split GFPSemantic proximity Read ↗

In vivo protein stabilization based on fragment complementation and a split GFP system.Semantic proximity Read ↗

Sensitive and Quantitative Three-Color Protein Imaging in Fission Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with Experimentally-Characterized In Vivo Maturation Kinetics.Semantic proximity Read ↗

Polyglutamine toxicity assays highlight the advantages of mScarlet for imaging in Saccharomyces cerevisiaeSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.