Article
A new protein-protein interaction sensor based on tripartite split-GFP association.
Scientific reports - 4 Oct 2013
Cabantous Stéphanie, Nguyen Hau B, Pedelacq Jean-Denis, Koraïchi Faten, Chaudhary Anu, Ganguly Kumkum, Lockard Meghan A, Favre Gilles, Terwilliger Thomas C, Waldo Geoffrey S
Abstract excerpt
Monitoring protein-protein interactions in living cells is key to unraveling their roles in numerous cellular processes and various diseases. Previously described split-GFP based sensors suffer from poor folding and/or self-assembly background fluorescence. Here, we have engineered a micro-tagging system to monitor protein-protein interactions in vivo and in vitro. The assay is based on tripartite association...
Topics
- Animals
- Cell Line
- Escherichia coli
- Gene Expression
- Gene Order
- Genetic Vectors
- Green Fluorescent Proteins
- Humans
- Molecular Imaging
- Mutation
- Protein Binding