Article
Structure-Based Rational Design of Two Enhanced Bacterial Lipocalin Blc Tags for Protein-PAINT Super-resolution Microscopy.
ACS chemical biology - 18 Sept 2020
Muslinkina Liya, Gavrikov Alexey S, Bozhanova Nina G, Mishin Alexander S, Baranov Mikhail S, Meiler Jens, Pletneva Nadya V, Pletnev Vladimir Z, Pletnev Sergei
Abstract excerpt
Super-resolution fluorescent imaging in living cells remains technically challenging, largely due to the photodecomposition of fluorescent tags. The recently suggested protein-PAINT is the only super-resolution technique available for prolonged imaging of proteins in living cells. It is realized with complexes of fluorogen-activating proteins, expressed as fusions, and solvatochromic synthetic dyes. Once...
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