Article
Rational design of a monomeric and photostable far-red fluorescent protein for fluorescence imaging in vivo.
Protein science : a publication of the Protein Society - 1 Feb 2016
Yu Dan, Dong Zhiqiang, Gustafson William Clay, Ruiz-González Rubén, Signor Luca, Marzocca Fanny, Borel Franck, Klassen Matthew P, Makhijani Kalpana, Royant Antoine, Jan Yuh-Nung, Weiss William A, Guo Su, Shu Xiaokun
Abstract excerpt
Fluorescent proteins (FPs) are powerful tools for cell and molecular biology. Here based on structural analysis, a blue-shifted mutant of a recently engineered monomeric infrared fluorescent protein (mIFP) has been rationally designed. This variant, named iBlueberry, bears a single mutation that shifts both excitation and emission spectra by approximately 40 nm. Furthermore, iBlueberry is four times more...
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