Article
A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules.
Cell - 8 Sept 2006
Banaszynski Laura A, Chen Ling-Chun, Maynard-Smith Lystranne A, Ooi A G Lisa, Wandless Thomas J
Abstract excerpt
Rapid and reversible methods for perturbing the function of specific proteins are desirable tools for probing complex biological systems. We have developed a general technique to regulate the stability of specific proteins in mammalian cells using cell-permeable, synthetic molecules. We engineered mutants of the human FKBP12 protein that are rapidly and constitutively degraded when expressed in mammalian cells,...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.