Article
Mutagenesis of mNeptune Red-Shifts Emission Spectrum to 681-685 nm.
PloS one - 1 Jan 2016
Li ZhaoYang, Zhang ZhiPing, Bi LiJun, Cui ZongQiang, Deng JiaoYu, Wang DianBing, Zhang Xian-En
Abstract excerpt
GFP-like fluorescent proteins with diverse emission wavelengths have been developed through mutagenesis, offering many possible choices in cellular and tissue imaging, such as multi-targets imaging, deep tissue imaging that require longer emission wavelength. Here, we utilized a combined approach of random mutation and structure-based rational design to develop new NIR fluorescent proteins on the basis of a...
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