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Article

Improved sensitivity of firefly luminescent intermediate-based protein interaction assay using Ser 440 mutant with lower adenylation activity.

Luminescence : the journal of biological and chemical luminescence - 1 Feb 2018

Ohmuro-Matsuyama Yuki, Yamashita Takahiro, Lin Huan, Yamaji Hideki, Ueda Hiroshi

Abstract excerpt

Protein-protein interaction assays are important in various fields including molecular biology, diagnostics, and drug screening. We recently designed a novel protein-protein interaction assay, the firefly luminescent intermediate-based protein interaction assay (FlimPIA), that exploited the unique reaction mechanism of firefly luciferase (Fluc). Using two mutant Flucs, each impaired with one of the two...

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PMID: 28752574
DOI: 10.1002/bio.3381

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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