Article
Generation of Nonmosaic, Two-Pore Channel 2 Biallelic Knockout Pigs in One Generation by CRISPR-Cas9 Microinjection Before Oocyte Insemination.
The CRISPR journal - 1 Feb 2021
Navarro-Serna Sergio, Hachem Alaa, Canha-Gouveia Analuce, Hanbashi Ali, Garrappa Gabriela, Lopes Jordana S, París-Oller Evelyne, Sarrías-Gil Lucía, Flores-Flores Cesar, Bassett Andrew, Sánchez Raul, Bermejo-Álvarez Pablo, Matás Carmen, Romar Raquel, Parrington John, Gadea Joaquín
Abstract excerpt
Studies of knockout (KO) mice with defects in the endolysosomal two-pore channels (TPCs) have shown TPCs to be involved in pathophysiological processes, including heart and muscle function, metabolism, immunity, cancer, and viral infection. With the objective of studying TPC2's pathophysiological roles for the first time in a large, more humanlike animal model, TPC2 KO pigs were produced using CRISPR-Cas9. A...
Topics
- Animals
- CRISPR-Cas Systems
- Calcium Channels
- Embryo Transfer
- Embryo, Mammalian
- Female
- Fertilization
- Fetus
- Gene Knockout Techniques