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Article

Molecular basis of nucleotide-dependent substrate engagement and remodeling by an AAA+ activator.

Nucleic acids research - 1 Aug 2014

Darbari Vidya C, Lawton Ed, Lu Duo, Burrows Patricia C, Wiesler Simone, Joly Nicolas, Zhang Nan, Zhang Xiaodong, Buck Martin

Abstract excerpt

Binding and hydrolysis of ATP is universally required by AAA+ proteins to underpin their mechano-chemical work. Here we explore the roles of the ATPase site in an AAA+ transcriptional activator protein, the phage shock protein F (PspF), by specifically altering the Walker B motif sequence required in catalyzing ATP hydrolysis. One such mutant, the E108Q variant, is defective in ATP hydrolysis but fully remodels...

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Identifiers

PMID: 25063294
DOI: 10.1093/nar/gku588
PMCID: PMC4132715

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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