Article
A nucleotide switch in the Escherichia coli DnaA protein initiates chromosomal replication: evidnece from a mutant DnaA protein defective in regulatory ATP hydrolysis in vitro and in vivo.
The Journal of biological chemistry - 26 Apr 2002
Nishida Satoshi, Fujimitsu Kazuyuki, Sekimizu Kazuhisa, Ohmura Tadahiro, Ueda Tadashi, Katayama Tsutomu
Abstract excerpt
The ATP-bound DnaA protein opens duplex DNA at the Escherichia coli origin of replication, leading to a series of initiation reactions in vitro. When loaded on DNA, the DNA polymerase III sliding clamp stimulates hydrolysis of DnaA-bound ATP in the presence of the IdaB/Hda protein, thereby yielding ADP-DnaA, which is inactive for initiation in vitro. This negative feedback regulation of DnaA activity is proposed...
Read the complete abstract on PubMedTopics
- Adenosine Triphosphate
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Cell Division