Article
Restoration of growth to acidic phospholipid-deficient cells by DnaA(L366K) is independent of its capacity for nucleotide binding and exchange and requires DnaA.
The Journal of biological chemistry - 18 Mar 2005
Li Zhenya, Kitchen Jennifer L, Boeneman Kelly, Anand Priyanka, Crooke Elliott
Abstract excerpt
In the absence of adequate levels of cellular acidic phospholipids, Escherichia coli remain viable but are arrested for growth. Expression of a DnaA protein that contains a single amino acid substitution in the membrane-binding domain, DnaA(L366K), in concert with expression of wild-type DnaA protein, restores growth. DnaA protein has high affinity for ATP and ADP, and in vitro lipid bilayers that are fluid and...
Topics
- Adenosine Diphosphate
- Adenosine Triphosphate
- Bacterial Proteins
- Cell-Free System
- Chromosomes
- Chromosomes, Bacterial
- DNA Replication
- DNA-Binding Proteins
- Dose-Response Relationship, Drug
- Escherichia coli
- Hydrolysis
- Lipid Bilayers