Article
A simplified transposon mutagenesis method to perform phenotypic forward genetic screens in cultured cells.
BMC genomics - 17 Jun 2019
Feddersen Charlotte R, Wadsworth Lexy S, Zhu Eliot Y, Vaughn Hayley R, Voigt Andrew P, Riordan Jesse D, Dupuy Adam J
Abstract excerpt
BACKGROUND: The introduction of genome-wide shRNA and CRISPR libraries has facilitated cell-based screens to identify loss-of-function mutations associated with a phenotype of interest. Approaches to perform analogous gain-of-function screens are less common, although some reports have utilized arrayed viral expression libraries or the CRISPR activation system. However, a variety of technical and logistical...
Topics
- Animals
- Cell Line
- DNA Transposable Elements
- Genetic Testing
- Humans
- Mutagenesis
- Mutagenesis, Insertional
- Phenotype
- Vemurafenib