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Validation-Based Insertional Mutagenesis (VBIM), A Powerful Forward Genetic Screening Strategy.

Current protocols - 1 Mar 2022

De Sarmishtha, Tamagno Ilaria, Stark George R, Jackson Mark W

Abstract excerpt

Forward genetics begins with a biological phenotype and attempts to identify genetic changes that influence that phenotype. These changes can be induced in a selected group of genes, for instance, by using libraries of cDNAs, shRNAs, CRISPR guide RNAs, or genetic suppressor elements (GSEs), or randomly throughout the genome using chemical or insertional mutagens, with each approach creating distinct genetic...

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PMID: 35316583
DOI: 10.1002/cpz1.394
PMCID: PMC8969887

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