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Genotype from Phenotype: Using CRISPR Screens to Dissect Lymphoma Biology.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2025

Bolomsky Arnold, Choi Jaewoo, Phelan James D

Abstract excerpt

Genome-wide screens are a powerful technique to dissect the complex network of genes regulating diverse cellular phenotypes. The recent adaptation of the CRISPR-Cas9 system for genome engineering has revolutionized functional genomic screening. Here, we present protocols used to introduce Cas9 into human lymphoma cell lines, produce high-titer lentivirus of a genome-wide sgRNA library, transduce and culture cells...

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PMID: 39424727
DOI: 10.1007/978-1-0716-4188-0_10

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