Article
Single-cell analysis of a mutant library generated using CRISPR-guided deaminase in human melanoma cells.
Communications biology - 2 Apr 2020
Jun Soyeong, Lim Hyeonseob, Chun Honggu, Lee Ji Hyun, Bang Duhee
Abstract excerpt
CRISPR-based screening methods using single-cell RNA sequencing (scRNA-seq) technology enable comprehensive profiling of gene perturbations from knock-out mutations. However, evaluating substitution mutations using scRNA-seq is currently limited. We combined CRISPR RNA-guided deaminase and scRNA-seq technology to develop a platform for introducing mutations in multiple genes and assessing the mutation-associated...
Topics
- Antineoplastic Agents
- Biomarkers, Tumor
- CRISPR-Cas Systems
- Cell Line, Tumor
- Clustered Regularly Interspaced Short Palindromic Repeats
- Cytidine Deaminase
- Female
- Gene Editing
- Gene Library
- HEK293 Cells