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Article

Batch Transduction of Transposon Mutant Libraries for Rapid Phenotype Screening in Staphylococcus.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2016

Maliszewski Katherine L

Abstract excerpt

In the gram-positive pathogen Staphylococcus aureus, transposon mutagenesis is a useful method of screening large numbers of mutants for a given phenotype. However, constructing a transposon mutant library can take several months of work and validation in the laboratory. In this chapter, we describe a method for batch transduction of existing transposon mutations into new genetic backgrounds. Transduction in S....

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PMID: 26194710
DOI: 10.1007/7651_2015_281

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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