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Article

Active site alanine mutations convert deubiquitinases into high-affinity ubiquitin-binding proteins.

EMBO reports - 1 Oct 2018

Morrow Marie E, Morgan Michael T, Clerici Marcello, Growkova Katerina, Yan Ming, Komander David, Sixma Titia K, Simicek Michal, Wolberger Cynthia

Abstract excerpt

A common strategy for exploring the biological roles of deubiquitinating enzymes (DUBs) in different pathways is to study the effects of replacing the wild-type DUB with a catalytically inactive mutant in cells. We report here that a commonly studied DUB mutation, in which the catalytic cysteine is replaced with alanine, can dramatically increase the affinity of some DUBs for ubiquitin. Overexpression of these...

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Identifiers

PMID: 30150323
DOI: 10.15252/embr.201745680
PMCID: PMC6172466

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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