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Article

MNase, as a probe to study the sequence-dependent site exposures in the +1 nucleosomes of yeast.

Nucleic acids research - 21 Aug 2018

Luo Di, Kato Daiki, Nogami Jumpei, Ohkawa Yasuyuki, Kurumizaka Hitoshi, Kono Hidetoshi

Abstract excerpt

The first nucleosomes in the downstream of transcription starting sites are called +1 nucleosomes, which are expected to be readily unwrapped for DNA transcription. To investigate DNA accessibility in +1 nucleosomes, MNase-seq experiments were carried out with 20 reconstituted +1 nucleosomes of budding yeast. Although MNase has been known for its sequence preference in DNA digestions, we confirmed that this...

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Identifiers

PMID: 29893974
DOI: 10.1093/nar/gky502
PMCID: PMC6101533

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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