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Article

High-throughput evaluation of T7 promoter variants using biased randomization and DNA barcoding.

PloS one - 1 Jan 2018

Komura Ryo, Aoki Wataru, Motone Keisuke, Satomura Atsushi, Ueda Mitsuyoshi

Abstract excerpt

Cis-regulatory elements (CREs) are one of the important factors in controlling gene expression and elucidation of their roles has been attracting great interest. We have developed an improved method for analyzing a large variety of mutant CRE sequences in a simple and high-throughput manner. In our approach, mutant CREs with unique barcode sequences were obtained by biased randomization in a single PCR...

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Topics

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Share to Bacteriophage T7
Share to DNA Barcoding, Taxonomic
Share to DNA-Directed RNA Polymerases

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Gene Expression Regulation

Share to High-Throughput Nucleotide Sequencing

Share to Promoter Regions, Genetic

Share to Regulatory Sequences, Nucleic Acid

Share to Transcription, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 29734387
DOI: 10.1371/journal.pone.0196905
PMCID: PMC5937735

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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