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Article

P3 site-directed mutagenesis: An efficient method based on primer pairs with 3'-overhangs.

The Journal of biological chemistry - 1 Mar 2025

Mousavi Negar, Zhou Ethan, Razavi Arezousadat, Ebrahimi Elham, Varela-Castillo Paulina, Yang Xiang-Jiao

Abstract excerpt

Site-directed mutagenesis is a fundamental tool indispensable for protein and plasmid engineering. An important technological question is how to achieve the ideal efficiency of 100%. Based on complementary primer pairs, the QuickChange method has been widely used, but it requires significant improvements due to its low efficiency and frequent unwanted mutations. An alternative and innovative strategy is to...

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Identifiers

PMID: 39863101
DOI: 10.1016/j.jbc.2025.108219
PMCID: PMC11910099

Genes

GC
KAT2B

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