Article
Selection of a T7 promoter mutant with enhanced in vitro activity by a novel multi-copy bead display approach for in vitro evolution.
Nucleic acids research - 7 Jan 2013
Paul Siddhartha, Stang Alexander, Lennartz Klaus, Tenbusch Matthias, Überla Klaus
Abstract excerpt
In vitro evolution of nucleic acids and proteins is a powerful strategy to optimize their biological and physical properties. To select proteins with the desired phenotype from large gene libraries, the proteins need to be linked to the gene they are encoded by. To facilitate selection of the desired phenotype and isolation of the encoding DNA, a novel bead display approach was developed, in which each member of...
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