Article
Dinucleotide tag-based parallel reporter gene assay method enables efficient identification of regulatory mutations.
Biotechnology journal - 1 Apr 2022
Ren Naixia, Li Bo, Liu Qingqing, Yang Lele, Liu Xiaodan, Huang Qilai
Abstract excerpt
BACKGROUND: The causal single nucleotide polymorphisms (SNPs) leading to increased cancer predisposition mainly function as gene regulatory elements, the evaluation of which largely relies on the parallel reporter gene assay system. However, the common DNA barcodes used in parallel reporter gene assay systems typically because nucleotide composition bias, and many barcodes must be allocated for each sequence to...
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