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Gene Expression and Transcription Factor Binding Tests Using Mutated-Promoter Reporter Lines.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018

Nishizawa-Yokoi Ayako, Yamaguchi Nobutoshi

Abstract excerpt

To control the expression of their target genes, plant transcription factors bind to specific DNA sequences (called cis-elements) adjacent to the genes they regulate, thereby promoting or blocking the recruitment of RNA polymerase. Functional analysis of cis-elements is therefore essential for understanding transcriptional regulation, which underlies developmental programs and environmental responses. Using...

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PMID: 30043377
DOI: 10.1007/978-1-4939-8657-6_17

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