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Article

The directed evolution of ligand specificity in a GPCR and the unequal contributions of efficacy and affinity.

Scientific reports - 22 Nov 2017

Di Roberto Raphaël B, Chang Belinda, Peisajovich Sergio G

Abstract excerpt

G protein-coupled receptors (GPCRs) must discriminate between hundreds of related signal molecules. In order to better understand how GPCR specificity can arise from a common promiscuous ancestor, we used laboratory evolution to invert the specificity of the Saccharomyces cerevisiae mating receptor Ste2. This GPCR normally responds weakly to the pheromone of the related species Kluyveromyces lactis, though we...

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Identifiers

PMID: 29167562
DOI: 10.1038/s41598-017-16332-2
PMCID: PMC5700115

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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