Article

Functional expression of opioid receptors and other human GPCRs in yeast engineered to produce human sterols

2021-05-14

Abstract excerpt

<h4>ABSTRACT</h4> The yeast Saccharomyces cerevisiae is a powerful tool for studying G protein-coupled receptors (GPCRs) as they can be functionally coupled to its pheromone response pathway. However, some exogenous GPCRs, including the mu opioid receptor, are non-functional in yeast, which may be due to the presence of the fungal sterol ergosterol instead of the animal sterol cholesterol. We engineered yeast to...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: a259ff03-cc41-5756-8f27-1f6881b8ef5f
DOI: 10.1101/2021.05.12.443385

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Functional expression of opioid receptors and other human GPCRs in yeast engineered to produce human sterolsDOI 10.1101/2021.05.12.443385Read

Directed evolution of G protein-coupled receptors in yeast for higher functional production in eukaryotic expression hostsSemantic proximity Read

Advances in yeast synthetic biology for human G protein–coupled receptor biology and pharmacologySemantic proximity Read

Engineered cell differentiation and sexual reproduction in probiotic and mating yeastsSemantic proximity Read

Serotonin GPCR-based biosensing modalities in yeastSemantic proximity Read

Modifying Expression Modes of Human Neurotensin Receptor Type 1 Alters Sensing Capabilities for Agonists in Yeast Signaling Biosensor.Semantic proximity Read

Generation of designer receptors exclusively activated by designer drugs (DREADDs) using directed molecular evolution.Semantic proximity Read

Reprogramming G protein coupled receptor structure and function.Semantic proximity Read

A Scalable, Multiplexed Assay for Decoding Receptor-Ligand InteractionsSemantic proximity Read

Distinguishing Critical, Beneficial, Neutral and Harmful Mutations Uncovered in the Directed Evolution of a Yeast Membrane ReceptorSemantic proximity Read

Development of a yeast bioassay to characterize G protein-coupled receptor kinases. Identification of an NH2-terminal region essential for receptor phosphorylation.Semantic proximity Read

Tuning up the right signal: chemical and genetic approaches to study GPCR functionsSemantic proximity Read

Artificial Intelligence Uncovers Evolutionarily Conserved Intracellular Allosteric Modulators of GPCR-Gα InterfaceSemantic proximity Read