Article

Distinguishing Critical, Beneficial, Neutral and Harmful Mutations Uncovered in the Directed Evolution of a Yeast Membrane Receptor

2020-08-05

Abstract excerpt

We present a reversion analysis of mutations introduced during the directed evolution of the yeast G-protein coupled receptor (GPCR) Ste2p to detect a peptide biomarker of chronic kidney disease. Two mutated receptors are analyzed in this study. Mutations acquired during directed evolution were reverted one at a time to the wild-type residue to assess the mutation’s contribution to receptor function. Mutations in...

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Literature Corpus work: a6c5a6d1-e93a-5b49-8e0c-51eeee117b03
DOI: 10.1101/2020.08.04.236505

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