Directed evolution of G protein-coupled receptors in yeast for higher functional production in eukaryotic expression hosts

Abstract excerpt

Despite recent successes, many G protein-coupled receptors (GPCRs) remained refractory to detailed molecular studies due to insufficient production yields, even in the most sophisticated eukaryotic expression systems. Here we introduce a robust method employing directed evolution of GPCRs in yeast that allows fast and efficient generation of receptor variants which show strongly increased functional production...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Monoclonal and Polyclonal Antibodies Research
• Share to Receptor Mechanisms and Signaling
• Share to Viral Infectious Diseases and Gene Expression in Insects