Article
Single-Step qPCR and dPCR Detection of Diverse CRISPR-Cas9 Gene Editing Events In Vivo.
G3 (Bethesda, Md.) - 5 Oct 2017
Falabella Micol, Sun Linqing, Barr Justin, Pena Andressa Z, Kershaw Erin E, Gingras Sebastien, Goncharova Elena A, Kaufman Brett A
Abstract excerpt
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-associated protein 9 (Cas9)-based technology is currently the most flexible means to create targeted mutations by recombination or indel mutations by nonhomologous end joining. During mouse transgenesis, recombinant and indel alleles are often pursued simultaneously. Multiple alleles can be formed in each animal to create significant...
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