Single-Step qPCR and dPCR Detection of Diverse CRISPR-Cas9 Gene Editing Events In Vivo.

doi:10.1534/g3.117.300123

Article

Single-Step qPCR and dPCR Detection of Diverse CRISPR-Cas9 Gene Editing Events In Vivo.

G3 (Bethesda, Md.) - 5 Oct 2017

Falabella Micol, Sun Linqing, Barr Justin, Pena Andressa Z, Kershaw Erin E, Gingras Sebastien, Goncharova Elena A, Kaufman Brett A

Abstract excerpt

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-associated protein 9 (Cas9)-based technology is currently the most flexible means to create targeted mutations by recombination or indel mutations by nonhomologous end joining. During mouse transgenesis, recombinant and ind...

Topics

Alleles
Animals
CRISPR-Cas Systems
Female
Gene Editing
Mice, Inbred C57BL
Oligonucleotides
Polymerase Chain Reaction

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