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Droplet digital PCR or quantitative PCR for in-depth genomic and functional validation of genetically altered rodents.

Methods (San Diego, Calif.) - 1 Jul 2021

Lindner Loic, Cayrou Pauline, Rosahl Thomas W, Zhou Heather H, Birling Marie-Christine, Herault Yann, Pavlovic Guillaume

Abstract excerpt

Gene targeting and additive (random) transgenesis have proven to be powerful technologies with which to decipher the mammalian genome. With the advent of CRISPR/Cas9 genome editing, the ability to inactivate or modify the function of a gene has become even more accessible. However, the impact of each generated modification may be different from what was initially desired. Minimal validation of mutant alleles from...

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PMID: 33838271
DOI: 10.1016/j.ymeth.2021.04.001

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