Article
Rapid Evaluation of CRISPR Guides and Donors for Engineering Mice.
Genes - 8 Jun 2020
McBeath Elena, Parker-Thornburg Jan, Fujii Yuka, Aryal Neeraj, Smith Chad, Hofmann Marie-Claude, Abe Jun-Ichi, Fujiwara Keigi
Abstract excerpt
Although the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/ CRISPR associated protein 9 (Cas9) technique has dramatically lowered the cost and increased the speed of generating genetically engineered mice, success depends on using guide RNAs and donor DNAs which direct efficient knock-out (KO) or knock-in (KI). By Sanger sequencing DNA from blastocysts previously injected with the same CRISPR...
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