Article
Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants.
BMC biology - 21 Jun 2018
Codner Gemma F, Mianné Joffrey, Caulder Adam, Loeffler Jorik, Fell Rachel, King Ruairidh, Allan Alasdair J, Mackenzie Matthew, Pike Fran J, McCabe Christopher V, Christou Skevoulla, Joynson Sam, Hutchison Marie, Stewart Michelle E, Kumar Saumya, Simon Michelle M, Agius Loranne, Anstee Quentin M, Volynski Kirill E, Kullmann Dimitri M, Wells Sara, Teboul Lydia
Abstract excerpt
BACKGROUND: Recent advances in clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) genome editing have led to the use of long single-stranded DNA (lssDNA) molecules for generating conditional mutations. However, there is still limited available data on the efficiency and reliability of this method. RESULTS: We generated conditional mouse alleles using lssDNA donor...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.