Article
The loss-of-allele assay for ES cell screening and mouse genotyping.
Methods in enzymology - 1 Jan 2010
Frendewey David, Chernomorsky Rostislav, Esau Lakeisha, Om Jinsop, Xue Yingzi, Murphy Andrew J, Yancopoulos George D, Valenzuela David M
Abstract excerpt
Targeting vectors used to create directed mutations in mouse embryonic stem (ES) cells consist, in their simplest form, of a gene for drug selection flanked by mouse genomic sequences, the so-called homology arms that promote site-directed homologous recombination between the vector and the target gene. The VelociGene method for the creation of targeted mutations in ES cells employs targeting vectors, called...
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