Article
Discovery of transgene insertion sites by high throughput sequencing of mate pair libraries.
BMC genomics - 14 May 2014
Srivastava Anuj, Philip Vivek M, Greenstein Ian, Rowe Lucy B, Barter Mary, Lutz Cathleen, Reinholdt Laura G
Abstract excerpt
BACKGROUND: Transgenesis by random integration of a transgene into the genome of a zygote has become a reliable and powerful method for the creation of new mouse strains that express exogenous genes, including human disease genes, tissue specific reporter genes or genes that allow for tissue specific recombination. Nearly 6,500 transgenic alleles have been created by random integration in embryos over the last...
Topics
- Alleles
- Animals
- Cluster Analysis
- DNA Transposable Elements
- DNA-Binding Proteins
- Gene Library
- Gene Transfer Techniques
- Genome
- High-Throughput Nucleotide Sequencing
- Mice
- Mice, Transgenic
- Recombination, Genetic
- Sequence Analysis, DNA
- Superoxide Dismutase