Article
Rapid and precise genotyping of transgene zygosity in mice using an allele-specific method.
Life science alliance - 1 Jun 2023
Yang Jianqi, DeVore Alison N, Fu Daniel A, Spicer Mackenzie M, Guo Mengcheng, Thompson Samantha G, Ahlers-Dannen Katelin E, Polato Federica, Nussenzweig Andre, Fisher Rory A
Abstract excerpt
Precise determination of transgene zygosity is essential for use of transgenic mice in research. Because integration loci of transgenes are usually unknown due to their random insertion, assessment of transgene zygosity remains a challenge. Current zygosity genotyping methods (progeny testing, qPCR, and NGS-computational biology analysis) are time consuming, prone to error or technically challenging. Here, we...
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