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Article

Rational design of a pH-insensitive cyan fluorescent protein CyPet2 based on the CyPet crystal structure.

FEBS letters - 1 Jun 2017

Liu Rui, Hu Xiao-Jian, Ding Yu

Abstract excerpt

The emission spectrum of widely used CyPet is pH-sensitive. In order to synthesize a pH-insensitive cyan fluorescent protein by rational design, we solved the crystal structures of CyPet under different pH conditions. The indole group of the CyPet chromophore adopts a cis-coplanar conformation in acidic and neutral conditions, while it converts to trans-coplanar under basic conditions. His148 and Glu222 play a...

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Source

Identifiers

PMID: 28504316
DOI: 10.1002/1873-3468.12678

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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