Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Crystal structure of a new cyan fluorescent protein and its hue-shifted variants.

Biochemistry - 16 Jun 2009

Kikuchi Akihiro, Fukumura Eiko, Karasawa Satoshi, Shiro Yoshitsugu, Miyawaki Atsushi

Abstract excerpt

Green fluorescent protein (GFP) based techniques are well established in molecular biology; however, the detailed mechanism for the fine-tuning of fluorescent colors remains unclear. Here, we report the cloning and crystal structure of a new cyan-emitting GFP-like protein, KCy. We also developed a mutant protein with a high folding efficiency (KCy-G4219: lambda(abs) = 453 nm; lambda(em) = 486 nm). X-ray...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Anthozoa
Share to Cloning, Molecular
Share to Crystallography, X-Ray

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Green Fluorescent Proteins

Share to Models, Molecular

Share to Protein Conformation

Share to Protein Folding

Share to Sequence Alignment

Source

Identifiers

PMID: 19402703
DOI: 10.1021/bi801658p

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Crystal structure of a new cyan fluorescent protein and its hue-shifted variants.PMID 19402703Read ↗

Crystal structure of the cyan fluorescent protein Cerulean-S175G.Semantic proximity Read ↗

Crystal Structure of Phototoxic Orange Fluorescent Proteins with a Tryptophan-Based Chromophore.Semantic proximity Read ↗

The molecular structure of green fluorescent protein.Semantic proximity Read ↗

Cyan Thermal Proteins Derived From Thermal Green Protein.Semantic proximity Read ↗

The single T65S mutation generates brighter cyan fluorescent proteins with increased photostability and pH insensitivity.Semantic proximity Read ↗

Mutagenesis of mNeptune Red-Shifts Emission Spectrum to 681-685 nm.Semantic proximity Read ↗

Synthesis and sequence optimization of GFP mutants containing aromatic non-natural amino acids at the Tyr66 position.Semantic proximity Read ↗

Crystal structures and mutational analysis of amFP486, a cyan fluorescent protein from Anemonia majano.Semantic proximity Read ↗

Turning up the Green Light.Semantic proximity Read ↗

Extended Stokes shift in fluorescent proteins: chromophore-protein interactions in a near-infrared TagRFP675 variant.Semantic proximity Read ↗

Juggling with fluorescent proteins: Spectrum and structural changes of the mCardinal2 variants.Semantic proximity Read ↗

[Chromoproteins of the green fluorescent protein family: properties and applications].Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.