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Article

Efficiently folding and circularly permuted variants of the Sapphire mutant of GFP.

BMC biotechnology - 22 May 2003

Zapata-Hommer Otilia, Griesbeck Oliver

Abstract excerpt

BACKGROUND: The green fluorescent protein (GFP) has been widely used in cell biology as a marker of gene expression, label of cellular structures, fusion tag or as a crucial constituent of genetically encoded biosensors. Mutagenesis of the wildtype gene has yielded a number of improved variants such as EGFP or colour variants suitable for fluorescence resonance energy transfer (FRET). However, folding of some of...

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Identifiers

PMID: 12769828
DOI: 10.1186/1472-6750-3-5
PMCID: PMC161811

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