Article
Role of histidine 148 in stability and dynamics of a highly fluorescent GFP variant.
Biochimica et biophysica acta - 1 Apr 2013
Campanini Barbara, Pioselli Barbara, Raboni Samanta, Felici Paolo, Giordano Immacolata, D'Alfonso Laura, Collini Maddalena, Chirico Giuseppe, Bettati Stefano
Abstract excerpt
The armory of GFP mutants available to biochemists and molecular biologists is huge. Design and selection of mutants are usually driven by tailored spectroscopic properties, but some key aspects of stability, folding and dynamics of selected GFP variants still need to be elucidated. We have prepared, expressed and characterized three H148 mutants of the highly fluorescent variant GFPmut2. H148 is known to be...
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