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Article

The S28H mutation on mNeptune generates a brighter near-infrared monomeric fluorescent protein with improved quantum yield and pH-stability.

Acta biochimica et biophysica Sinica - 1 Sept 2014

Li Zhao-Yang, Wang Dian-Bing, Zhang Zhi-Ping, Bi Li-Jun, Cui Zong-Qiang, Deng Jiao-Yu, Zhang Xian-En

Abstract excerpt

For living deep-tissue imaging, the optical window favorable for light penetration is in near-infrared wavelengths, which requires fluorescent proteins with emission spectra in the near-infrared region. Here, we report that a single mutant Ser28His of mNeptune with a near-infrared (≥650 nm) emission maxima of 652 nm is found to improve the brightness, photostability, and pH stability when compared with its...

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PMID: 25062707
DOI: 10.1093/abbs/gmu063

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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